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产品简介

转录组测序(RNA-Seq)的研究对象是特定细胞在某一功能状态下所能转录出来的所有mRNA的总和。新一代高通量测序技术能够全面快速的获得某一物种特定组织或器官在某一状态下的几乎所有转录本序列信息,从而准确地分析基因表达差异、基因结构变异、筛选分子标记(SNPs或SSR)等生命科学重要问题。

A workflow for RNA seq

Ruairi J, Genomics Research, 2018


MG纯银3D多重奖励的优势

1、八年转录组测序分析经验,自主研发了多个国际认可的软件,如差异可变剪接算法ASD、CASH等,检出率和准确度超过同类软件;
2、不依赖已有物种信息,可研究非模式物种,针对不同平台的数据,制定多套流程;
3、整合了众多学术界公认的转录组相关数据库,从本质上提高后期分析的广度和精度。

样本要求

组织样品
动物组织≥1g;植物组织≥2g;细胞样品≥1×106个;全血≥2mL;菌体≥106个或≥30mg;
RNA样品
样品需求量: RNA≥10 μg;样品浓度:RNA样品≥100 ng/μl;样品纯度:OD260/OD280在1.8-2.2之间,OD260/OD230≥2,28S/18S≥1,动物样品RIN≥7.0,植物样品RIN≥6.5,RNA无明显降解。

实验流程


1. 客户样本:保证细胞量在10^6个以上,否则则需风险建库;
2. RNA提取:经典试剂盒快速提取法;
3. RNA质控:凝胶电泳质控→Nanodrop质控→Agilent2200质控;
4. 文库构建:polyA建库;
5. 上机测序:PT角斗士老虎机试玩建议选择NovaSeq测序平台,双端测序,通量大,碱基精度高,且成本低,速度快。推荐数据量:6Gb。

数据分析流程




结果示例

高级数据分析

文献示例

[1] Ju L, Han J, Zhang X, et al. Obesity-associated inflammation triggers an autophagy-lysosomal response in adipocytes and causes degradation of perilipin 1. Cell Death Dis. 2019 Feb 11;10(2):121. (IF=5.683)

[2] He H, Chen E, Lei L, et al. Alteration of the tumour suppressor SARDH in sporadic colorectal cancer: a functional and transcriptome profiling-based study. Mol Carcinog. 2019 Jan 29. (IF=3.851)

[3] Zhang C, Wang JJ, He X, et al. Characterization and Beige Adipogenic Potential of Human Embryo White Adipose Tissue-Derived Stem Cells. Cell Physiol Biochem. 2018 Dec 14;51(6):2900-2915. (IF=5.5)

[4] Chen E, Yang F, He H, et al. Alteration of tumor suppressor BMP5 in sporadic colorectal cancer: a genomic and transcriptomic profiling based study. Molecular Cancer. 2018 Dec 20; 17(1):176-188. (IF=7.776)

[5] Ge X, Chen J, Li L, et al. Midostaurin potentiates rituximab antitumor activity in Burkitt's lymphoma by inducing apoptosis. Cell Death Dis. 2018 Dec 18;10(1):8-19. (IF=5.638)

[6] Miao N, Bian S, Lee T, et al. Opposite Roles of Wnt7a and Sfrp1 in Modulating Proper Development of Neural Progenitors in the Mouse Cerebral Cortex. Front Mol Neurosci. 2018 Jul 17; 11:247-260. (IF=3.903)

[7] Heng S, Yan W, Zongyou P, et al. Gefitinib for Epidermal Growth Factor Receptor Activated Osteoarthritis Subpopulation Treatment. EBioMedicine. 2018 Jun 11;32:223-233. (IF=6.183)

[8] He c,et al. Phosphorylation of ETS-1 is a critical event in DNA polymerase iota-induced invasion and metastasis of esophageal squamous cell carcinoma. Cancer Sci. 2017 Sep 14. (IF=3.974)

[9] Wei, J. et al. The GARP Complex Is Involved in Intracellular Cholesterol Transport via Targeting NPC2 to Lysosomes. Cell Rep. 2017 Jun;19(13):2823-2835.(IF=8.032)

[10] Wu, W. et al. CASH: a constructing comprehensive splice site method for detecting alternative splicing events. Brief Bioinform. 2017 Feb;1-13. doi:10.1093/bib/bbx034 (IF=5.134)

[11] Chen J, et al. Network analysis-based approach for exploring the potential diagnostic biomarkers of acute myocardial infarction. Front Physiol. 2016 Dec 9;7:615.  (IF=3.394)

[12] Liu Z,et al.Autism-like behaviours and germline transmission in transgenic monkeys overexpressing MeCP2. Nature. 2016 Feb 4;530(7588):98-102. (IF=41.577)

[13] Hu, Y. et al. Interactions of OsMADS1 with floral homeotic genes in rice flower development. Mol. Plant 2015 Sep;8(9):1366-1384 (IF=8.827)

[14] Wang F, et al. Alternative splicing of the androgen receptor in polycystic ovary syndrome. Proc Natl Acad Sci U S A. 2015 Apr 14;112(15):4743-8. (IF=9.681)